Molecular Techniques for Testing Genetic Purity and Seed Health

Abstract With the globalization of seed trade and transgenic variety development, application molecular technologies for quality gained more significance in both internal international markets. Besides germination, genetic purity health are two most important parameters that determine planting value a lot. Compared to conventional methods testing, marker efficient analysis as these accurate, robust, abundant, faster. Among various markers, simple sequence repeats (SSRs), due their genome-wide presence, reproducibility, multi-allelic nature, co-dominant inheritance, have emerged best establishing varietal distinctness, identity, variety/hybrid testing. advent next-generation sequencing (NGS) technology, single nucleotide polymorphic (SNP) markers also became widely popular, closest being an ideal besides SSRs, large-scale GM crop cultivation, testing adventitious presence trait added components The include DNA-based (conventional real-time PCR), protein-based (lateral flow test ELISA), bioassay-based technologies. including PCR/real-time PCR assays been successfully employed detect seeds especially at level, well national gene banks germplasm conservation. ISTA plays prominent role harmonization providing universal guidelines on use different seeds. BMT group UPOV Working Group DNA Methods Variety Committee ISTA, work tandem standardize suitable techniques identity respectively. In area also, detection such as, (nested PCR, multiplex loop-mediated isothermal amplification (LAMP), microarray with many advantages over proven highly useful. However, there is need validate usefulness through stringent multi-laboratory tests reproducibility before recommending them routine